FINE STRUCTURE OF LIPID-DEPLETED MITOCHONDRIA

Fine Structure of Lipid-depleted Mitochondria

The fine structure of mitochondria and submitochondrial vesicles depleted of their lipid by extraction with aqueous acetone was studied. Thin sections of mitochondrial membranes depleted of more than 95% of their lipid retained the unit membrane structure. Densitometer tracings of the electron micrographs showed that the unit membrane of extracted mitochondria was, on the average, wider than th...

Fine Structure of Negatively Stained Plant Mitochondria

Recently investigators have reported knob-like particles on mitochondrial membranes isolated from different organisms (1, 2, 4, 5). This report presents observations on the structure of plant mitochondria, negatively stained with phospho-tungstic acid, which show knob-like projections on the membranes. MATERIALS AND METHODS Castor beans (Ricinus communis) were soaked for 24 hours in distilled w...

Deprotonation of lipid-depleted bacteriorhodopsin.

The removal of 75% of the lipid from bacteriorhodopsin caused the following: (i) decreased efficiency and rate of deprotonation of the protonated Schiff base (as monitored by absorption of the M412 intermediate); (ii) increased efficiency of deprotonation of deionized samples; (iii) a decrease by 1 unit in the pH at which deprotonation ceases; (iv) increased intensity of Eu3+ emission in Eu3+-r...

toxicity of depleted uranium on isolated rat kidney mitochondria

Toxicity of depleted uranium on isolated rat kidney mitochondria.

BACKGROUND Kidney is known as the most sensitive target organ for depleted uranium (DU) toxicity in comparison to other organs. Although the oxidative stress and mitochondrial damage induced by DU has been well investigated, the precise mechanism of DU-induced nephrotoxicity has not been thoroughly recognized yet. METHODS Kidney mitochondria were obtained using differential centrifugation fro...